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Main page Lab Homepage Curriculum vitae Publications Projects: Neurofibromin Glioma Invasion Glioma Motility (Movies) Laminin and motility Phd Dissertation Personal: SPETSES 2000 ERDÉLY 2001
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Glioma cell motility in vitro The highly malignant glioblastoma tumors are very diverse. However, under normal cell culture condition their proliferation rate is rather similar. In order to capture the inherent differences in the motility of cells we performed long-term videomicroscopic measurements with several glioma cell lines. In contrast to proliferation we found significant variation in cell motility among the cell lines established from different patients. Hegedus et al, 2000 Next we sought to determine whether therapeutic modalitites currently employed in neurooncological practice alter the in vitro motility of human glioma cell lines. Surprisingly, taxol treatment or low dose radiation as a monotherapy resulted in increased cell locomotion. However, combination treatment with the application of taxol prior to radiation resulted in a significant decrease in cell motility. Hegedus et al, 2004 Movies show cells from three different Glioblastoma multiformae (GBM) tumor. These tumors are known for their diversity, here differences in motility are apparent (in the order of increasing velocities): Specimen HC : 6MB mpeg stream. Objective: 10X (Field of view: 740um X 560um). Duration of recording: 3 days. (6 min/frame). Specimen HA : 22MB mpeg stream. Objective: 10X (Field of view: 740um X 560um). Duration of recording: 4 days. (6 min/frame). Specimen HB : 10MB mpeg stream. Objective: 10X (Field of view: 740um X 560um). Duration of recording: 3 days. (6 min/frame). Note the streaming behavior in the case of the HB specimen. |