Hegedûs B, Czirók A, Fazekas I#,
Bábel T#, Madarasz
E$ and
Vicsek T:
Locomotion and proliferation of glioblastoma cells in vitro:
statistical evaluation of videomicroscopic observations
J Neurosurgery
92(3):428-434, 2000
# National Institute of Neurosurgery, Budapest
$ Institute of Experimental Medicine, Hungarian Academy of
Sciences, Budapest
OBJECT: The motility and doubling of human glioblastoma cells were investigated
by means of statistical evaluation of large sets of data obtained using
computer-aided videomicroscopy.
METHODS: Data were obtained on cells in four
established glioblastoma cell lines and also on primary tumor cells cultured
from fresh surgical samples. Growth rates and cell cycle times were measured in
individual microscopic fields. The averages of cell cycle time and the
duplication time for the recorded cell populations were 26.2 +/- 5.6 hours and
38 +/- 4 hours, respectively. With these parameters, no significant differences
among the cell lines were revealed. Also, there was no correlation in the cell
cycle time of a parent cell and its progeny in any of the cultures. Statistical
analysis of cell locomotion revealed an exponential distribution of cell
velocities and strong fluctuations in individual cell velocities across time.
The average velocity values ranged from 4.2 to 27.9 micro/hour. In spite of the
uniform histopathological classification of the four tumors, each cell line
produced by these tumors displayed distinct velocity distribution profiles and
characteristic average velocity values. A comparison of recently established
primary cultures with cell lines that had propagated multiple times indicated
that cells derived from different tumors sustain their characteristic locomotor
activity after several passages.
CONCLUSIONS: It can be inferred from the data
that statistical evaluation of physical parameters of cell locomotion can
provide additional tools for tumor diagnosis.
pdf
Movies show cells from five different Glioblastoma multiformae (GBM)
tumor. These tumors are known for their diversity, here differences in
motility are apparent (in the order of increasing velocities):
Specimen HC : 0.5MB
mpeg stream.
Objective: 10X (Field of view: 740um X 560um). Duration
of recording: 1 days. Number of frames: 300 (6 min/frame).
Specimen HA : 1.5MB mpeg stream.
Objective: 10X (Field of view: 740um X 560um). Duration
of recording: 1.5 days. Number of frames: 370 (6 min/frame).
Specimen HB : 1.1MB mpeg stream.
Objective: 10X (Field of view: 740um X 560um). Duration
of recording: 2 days. Number of frames: 430 (6 min/frame).
Note the streaming behavior in the case of the HB specimen.
Specimen HF : 6.1MB
mpeg stream.
Objective: 20X (Field of view: 500um X 370um). Duration
of recording: 3 days. Number of frames: 852 (5 min/frame).
Specimen U87 : 5.3MB mpeg stream.
Objective: 20X (Field of view: 500um X 370um). Duration
of recording: 4 days. Number of frames: 1140 (5 min/frame).