Long-term automated live cell microscopy
Tissue cells, the alive building blocks of organisms, exhibit
properties: they syntetize a vast variety of chemicals, gather and
process information about their surroundings, communicate, move across
rugged environments or alter their biochemical composition. Advances in
microscopy, culturing techniques, molecular probes and
computing made possible the current revival of live cell
microscopy: to see the cells in action. The significance of the method
lies in the twofold possibilities, to reveal the dynamics of biological
changes, and to yield a large array of quantitative data.
Our live cell imaging stations consists automated
microscopes, capable for parallel
monitoring of a number of specimens for an extended period of time.
These long-term observations form the basis of obtaining cell
motion and cell division statistics from a large number of cells,
or to trace
developmental processes such as neuronal differentiation. We keep
improving the culturing techniques and also devote efforts to develop
new ways of statistical data extraction from time-lapse image sequences.
The laboratory was initiated by
Since then, a number of students and department staff made substantial
contribution to its development, which was first managed by
and later by
Various optical elements were
designed by Otto Haiman. Microscope and incubator controller electronics
was created by Miklos Csiszer, Zoltan Csahok and
Controller software was written by
parts were machined by Sandor Hopp and Endre Berecz. Tissue culture expertise
was provided by
Emilia Madarasz, Katalin Schlett, Zsuzsanna Kornyei,
and Elod Mehes.
cell motion statistics
astroglia/stem cell interactions